Improve Lab Efficiency: A Guide to Selecting Premium Serological Pipette Tips

How to Use the Serological Pipette and Its Tips Correctly to Improve Experimental Efficiency?

The key to using Serological Pipette and Tips correctly is: choose sterile, RNA/DNAase-free pre-sterilized conical tips that match the sealing of the pipette, pay attention to the adaptability of 5ml/10ml and other specifications to centrifuge tubes/culture bottles; use the forward aspiration method (pre-wet the inner wall of the Tips first), keep the pipette vertical, touch the tube wall lightly when dispensing, and tilt 45° to reduce liquid impact when pipetting 10ml large volume; for viscous samples, it is recommended to use low-absorption Tips and set the reverse aspiration mode. High-quality polypropylene material and precise taper design can ensure ±2% pipetting accuracy. It is recommended to use Tips with filter cartridges to prevent cross contamination - these details can improve the repeatability and efficiency of experiments such as cell culture and ELISA.

Two Misunderstandings to Avoid When Using the Serological Pipette and Their Tips Solutions

Myth 1: Thinking that pipette tips can be mixed at will - the taper of pipettes and tips of different brands may not match, resulting in poor sealing, leakage or volume error (it is recommended to choose original or certified compatible pre-sterilized low-adsorption tips);

Myth 2: Quick pressing operation can improve efficiency - sucking and releasing liquid too quickly can easily produce bubbles or residues (Solution: Use reverse aspiration mode for viscous liquids, keep a 45° tilt and release slowly when pipetting large volumes).

Tip: For corrosive liquids, chemically resistant tips should be used, and aseptic operations such as cell culture should use filter tips to avoid aerosol contamination.

What Are Pipette Tips? How to Choose High-Quality Tips for the Serological Pipette?

Pipette Tips are disposable consumables installed on the pipette for precise aspiration and transfer of liquids. They are usually made of polypropylene (PP) and have different capacities (such as 1mL, 5mL, 10mL) and designs (such as ordinary tips, filter tips, low-adsorption tips, etc.), suitable for biochemical experiments, cell culture, molecular biology and other fields.

How to choose high-quality Tips for Serological Pipette?

Serological Pipette is mainly used for pipetting of larger volumes (1mL~50mL) of liquids. The key points for purchasing its matching Tips include:

1. Compatibility

Taper adaptation: There may be differences in the interfaces of Serological Pipette tips of different brands (such as Corning, Eppendorf, Thermo Fisher). It is necessary to ensure that the tips can fit tightly to prevent leakage or poor exhaust (original or internationally certified compatible tips are recommended).

Capacity matching: The tip capacity must cover the calibration range of the pipette (e.g., 10mL Tips should be selected for a 10mL Pipette).

2. Material and surface treatment

Low retention: Reduce residues and improve recovery rates for precious samples (e.g., proteins, DNA).

Chemical tolerance: For strong acids/alkalis or organic solvents (e.g., DMSO, Triton X-100), high-purity polypropylene tips should be used to avoid dissolution of impurities.

3. Sterilization and biosafety

Pre-sterilized: For cell culture or sterile experiments, gamma-irradiated sterilized tips should be selected, and check whether the packaging is sealed (e.g., independent packaging or boxed with filter membrane).

Filter tips: Prevent aerosols from contaminating the inside of the pipette, suitable for pathogen detection or volatile reagents.

4. Experimental scenario adaptation

High precision requirements: Select tips with high precision classification (e.g., ASTM standards) and good transparency to facilitate observation of the liquid surface.

Automation compatibility: Automated pipetting workstations must be compatible with flat-mouth tips (such as Robotics Tips) to avoid slot errors.

The Difference Between the Pipette and the Serological Pipette: Why You Need Dedicated Tips?

The core difference between Pipette and Serological Pipette

Pipette (traditional pipette) is mostly used for small volume (μLmL) precise pipetting, and requires manual change of adjustable range, while Serological Pipette (serological pipette) is designed for large volume (150mL) liquid, usually fixed range, disposable, relying on electronic control or mechanical pipette (such as Pipet-Aid) operation.

Why do you need special Tips?

1️⃣ Higher sealing requirements: Serological Pipette tips have a wider taper, and ordinary tips are prone to leaking (such as 100% airtightness for 10mL pipetting);

2️⃣ Anti-deformation ability: Large volume liquid has greater negative pressure, and low-quality tips may collapse (high-quality tips use thickened polypropylene);

3️⃣ Sterility and biosafety: Serological experiments often involve cell culture or serum, and filter tips need to be pre-sterilized to avoid contamination.