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Minimum Volume for 96 Well Plate
The capacity of each well of a 96-well plate can vary according to the design of different manufacturers, but in general, the capacity of each well of a standard 96-well plate ranges from 100 microliters to 365 microliters. Among them, 250 microliters is the most common capacity, which means that at least 100 microliters of culture medium or experimental reagent can be added to each well. This design not only helps to save precious experimental materials, but also allows high-throughput experimental operations in a limited space, thereby improving experimental efficiency.
The minimum capacity selection of a 96-well plate needs to consider the specific needs of the experiment.
For example, in a cell culture experiment, the number of cells per well and the volume of culture medium need to be optimized according to the growth characteristics of the cells and the purpose of the experiment. Usually, 96 well plates have no less than 104 cells per well and per milliliter of culture medium to ensure that the cells have sufficient growth space and nutritional support. If only one cell is inoculated per well, the volume of the culture medium can be reduced accordingly, but generally not less than 100 microliters to ensure the basic living conditions of the cells.
The material and bottom shape of the 96-well plate will also affect the choice of capacity.
Common bottom shapes include flat bottom and round bottom (U-shaped and V-shaped), and different bottom shapes are suitable for different experimental purposes. For example, the flat bottom is suitable for general cell culture, while the U-shaped bottom and V-shaped bottom are more suitable for certain specific cell separation and precipitation experiments.
In terms of material, polystyrene is the most common choice because of its good optical transparency and cell affinity.
During the experimental operation, in order to ensure the accuracy and uniformity of the experimental results, the uniform distribution of inoculated cells is crucial. It is recommended to use a multichannel pipette for sample addition to avoid uneven wells caused by adding one by one. At the same time, do not shake or knock the plate after adding the cell suspension, but put it directly into the incubator to let it adhere naturally to the wall to ensure the uniform distribution of cells in the well.
