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Considerations for Choosing Wide Bore Tips
Pipette tips are commonly used tools in the fields of life sciences, pharmaceutical research, and molecular biology, but the diversity of experiments often poses challenges for researchers in choosing the right pipette tips. Selecting the appropriate pipette tips is crucial for ensuring the success of scientific experiments. When choosing tips, researchers must comprehensively consider multiple specialized points to ensure the smoothness and accuracy of the experimental process and the reliability of the final data.
What are wide bore tips?
Wide bore tips are designed for pipetting challenging and delicate samples. Compared to regular tips, they have a larger opening, making it easier to dispense and providing less mechanical shearing. In terms of application, wide bore tips are ideal for handling viscous materials, genomic DNA, and cell culture fluids.
Applications of wide bore tips
Macromolecular experiments
e.g.: Genomic DNA research.
Delicate cell samples
e.g.: Macrophages, hybridoma cells, hepatocytes, and other cell samples.
Viscous liquids
e.g.: Glycerol, polyacrylic acid.
Key points for selecting wide bore tips
Tip capacity
Select the appropriate capacity of wide bore tips based on the required pipetting volume of the experiment. Common tip capacities include 10µL, 100uL, 200µL, 1000µL, etc. It is important to avoid using tips outside the suitable capacity range to prevent compromising pipetting accuracy.
Compatibility with pipettes
The compatibility of wide bore tips with pipettes is key to ensuring pipetting accuracy. Different brands of pipettes may require specific types of tips. When purchasing, it is essential to confirm whether the tips are compatible with existing pipettes, primarily by evaluating the seal between the pipette and the tip, which can usually be identified more easily by using a discharge gun.
Quality and manufacturing process
High-quality wide bore tips undergo stringent sterile processing during production, have smooth inner walls with no residues, and can effectively reduce sample loss. Additionally, the manufacturing process determines the consistency between batches. During experiments, it is advisable to use tips from the same batch whenever possible, and multiple tests can help determine the consistency of results.
General biological and medical diagnostic tests require that the consumables used are free of enzymes and endotoxins, and have high standards of cleanliness. The materials generally must pass biocompatibility or toxicity tests.
Storage and use
Wide bore tips should be stored in a dry, cool environment, avoiding direct sunlight and high temperatures to prevent material aging, which can affect performance. Additionally, during use, it is important to avoid secondary contamination and maintain the cleanliness of the tips.
Why choose wide bore tips from Eakan?
Wide bore tips provide comprehensive support for experiments, whether for macromolecular experiments or the handling of delicate cell samples. Eakan's wide bore tips not only guarantee high quality but also offer better operability and experimental results, making your experiments easier and more efficient!
